The Morphology and Composition of Influenza A Virus Particles Are Not Affected by Low Levels of M1 and M2 Proteins in Infected Cells
Open Access
- 15 June 2005
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 79 (12) , 7926-7932
- https://doi.org/10.1128/jvi.79.12.7926-7932.2005
Abstract
We generated a recombinant influenza A virus (Mmut) that produced low levels of matrix (M1) and M2 proteins in infected cells. Mmut virus propagated to significantly lower titers than did wild-type virus in cells infected at low multiplicity. By contrast, virion morphology and incorporation of viral proteins and vRNAs into virus particles were similar to those of wild-type virus. We propose that a threshold amount of M1 protein is needed for the assembly of viral components into an infectious particle and that budding is delayed in Mmut virus-infected cells until sufficient levels of M1 protein accumulate at the plasma membrane.Keywords
This publication has 39 references indexed in Scilit:
- Influenza B Virus BM2 Protein Is a Crucial Component for Incorporation of Viral Ribonucleoprotein Complex into Virions during Virus AssemblyJournal of Virology, 2004
- Introduction of a Temperature-Sensitive Phenotype into Influenza A/WSN/33 Virus by Altering the Basic Amino Acid Domain of Influenza Virus Matrix ProteinJournal of Virology, 2004
- Basic Residues of the Helix Six Domain of Influenza Virus M1 Involved in Nuclear Translocation of M1 Can Be Replaced by PTAP and YPDL Late Assembly Domain MotifsJournal of Virology, 2003
- Reverse genetics studies on the filamentous morphology of influenza A virusJournal of General Virology, 2003
- Alternative base pairs attenuate influenza A virus when introduced into the duplex region of the conserved viral RNA promoter of either the NS or the PA geneJournal of General Virology, 2003
- Influenza A Virus M2Ion Channel Activity Is Essential for Efficient Replication in Tissue CultureJournal of Virology, 2002
- Influenza A Virus Can Undergo Multiple Cycles of Replication without M2 Ion Channel ActivityJournal of Virology, 2001
- Influenza Virus Matrix Protein Is the Major Driving Force in Virus BuddingJournal of Virology, 2000
- Influenza virus M2 protein has ion channel activityPublished by Elsevier ,1992
- Nuclear transport of influenza virus ribonucleoproteins: The viral matrix protein (M1) promotes export and inhibits importCell, 1991