Owades and Jakovac Method for Diacetyl Determination in Mixed-Strain Starters

Abstract
A method for the determination of dlacetyl in beer was applied to single- and mixed-strain lactic streptococcus starter cultures of S. lactls, diacetilactls. Samples (20 ml) in a 65[degree]C water bath were purged with N gas and the emerging diacetyl trapped as di-methylglyoxime in buffered hydroxylamlne and converted to a colored complex by reaction with ferrous-sulfate. Advantages of the method over 2 other procedures include greater accuracy and sensitivity and use of simple inexpensive equipment.

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