Evidence for different binding sites on the 33‐kDa protein for DCMU, atrazine and QB
- 27 February 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 167 (2) , 321-326
- https://doi.org/10.1016/0014-5793(84)80150-7
Abstract
Two DCMU‐resistant strains of the cyanobacteriumSynechocystis6714 were used to analyse the binding sites of DCMU, atrazine and QB. DCMUr‐IIAwas DCMU and atrazine resistant; it presented an impaired electron flow and its 33‐kDa protein was weakly attached to the membrane. DCMUr‐IIB, derived from the former, simultaneously regained atrazine sensitivity, normal electron flow and a tight linkage of the 33‐kDa protein to the membrane. This mutant shows that loss of DCMU binding does not necessarily affect the binding of either atrazine or QB. The role of the 33‐kDa protein is discussed.Keywords
This publication has 20 references indexed in Scilit:
- Interaction of exogenous benzoquinone with Photosystem II in chloroplasts. The semiquinone form acts as a dichlorophenyldimethylurea-insensitive secondary acceptorBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1982
- Identification of the triazine receptor protein as a chloroplast gene productProceedings of the National Academy of Sciences, 1981
- Characterization and function of a 33 000 Mr polypeptide in DCMU‐sensitive and resistant strains of a cyanobacterium, Aphanocapsa 6714FEBS Letters, 1981
- Properties of the proteinaceous component acting as apoenzyme for the functional plastoquinone redox groups on the acceptor side of system IIBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1981
- Electron‐dependent competition between plastoquinone and inhibitors for binding to photosystem IIFEBS Letters, 1981
- The rapidly metabolized 32,000-dalton polypeptide of the chloroplast is the "proteinaceous shield" regulating photosystem II electron transport and mediating diuron herbicide sensitivity.Proceedings of the National Academy of Sciences, 1981
- Photoaffinity labelling of the photosystem II herbicide binding proteinFEBS Letters, 1980
- Modification of Herbicide Binding to Photosystem II in Two Biotypes of Senecio vulgaris LPlant Physiology, 1979
- Loss of sensitivity to diuron after trypsin digestion of chloroplast photosystem II particlesFEBS Letters, 1979
- Relationship between inhibitor binding by chloroplasts and inhibition of photosynthetic electron transportBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1977