Isolation and characterization of the murine lymphoma L5178Y cell line highly resistant to 5-fluorouracil.
- 1 January 1980
- journal article
- research article
- Published by Pharmaceutical Society of Japan in Journal of Pharmacobio-Dynamics
- Vol. 3 (8) , 390-394
- https://doi.org/10.1248/bpb1978.3.390
Abstract
5-Flourouracil-resistant cell line designated as L5178Y/FU was established in this experiment. This is one of the colonies derived from the subculture which acquired resistance to 5-flourouracil by passaging the [mouse lymphoma] L5178Y cells through 10 successive episodes of culture in Fischer''s medium containing 5-flourouracil, in which each 5-flourouracil treatment was followed by recovery intervals. The resistance of this line is about 80-fold that of the IC99 [concentration required for 99% inhibition] of 5-flourouracil, and shows a cross resistance to 5-flourouridine and 5-fluoro-2''-deoxyuridine. 5-Fluoro-2''-deoxyuridine resistant subline designated as L5178Y/FUdR was also isolated from the subculture which acquired resistance to 5-fluoro-2''-deoxyuridine by using the same selection procedure. The resistance of this line is about 650-fold that of the IC99 of 5-fluoro-2''-deoxyuridine, and shows a partial cross-resistance to 5-fluorouridine, but not to 5-fluorouracil.This publication has 0 references indexed in Scilit: