Radioprotection by glutathione ester: transport of glutathione ester into human lymphoid cells and fibroblasts.
- 1 August 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (15) , 4732-4735
- https://doi.org/10.1073/pnas.81.15.4732
Abstract
Glutathione is not effectively transported into human lymphoid cells, normal human skin fibroblasts and fibroblasts from patients with genetic deficiencies of .gamma.-glutamylcysteine synthetase or glutathione synthetase. The monoethyl ester of glutathione, in which the carboxyl group of the glycine residue is esterified, is readily transported into these cells and is hydrolyzed intracellularly. This leads to greatly increased cellular levels of glutathione, which often exceed those found normally. Glutathione ester protected human lymphoid cells of the CEM line against the lethal effects of irradiation. Under the conditions employed, complete protection was found when the ester was added prior to irradiation. Addition of the ester after irradiation was partially effective, suggesting that GSH may also function in repair processes.This publication has 17 references indexed in Scilit:
- Potent and specific inhibition of glutathione synthesis by buthionine sulfoximine (S-n-butyl homocysteine sulfoximine).Published by Elsevier ,2021
- Glutathione export by human lymphoid cells: depletion of glutathione by inhibition of its synthesis decreases export and increases sensitivity to irradiation.Proceedings of the National Academy of Sciences, 1981
- Stimulation of hepatic glutathione formation by administration of L-2-oxothiazolidine-4-carboxylate, a 5-oxo-L-prolinase substrate.Proceedings of the National Academy of Sciences, 1981
- Inhibition of glutathione biosynthesis by prothionine sulfoximine (S-n-propyl homocysteine sulfoximine), a selective inhibitor of gamma-glutamylcysteine synthetase.Journal of Biological Chemistry, 1979
- Evidence that the gamma-glutamyl cycle functions in vivo using intracellular glutathione: effects of amino acids and selective inhibition of enzymes.Proceedings of the National Academy of Sciences, 1978
- Regulation of gamma-glutamyl-cysteine synthetase by nonallosteric feedback inhibition by glutathione.Journal of Biological Chemistry, 1975
- Familial Spinocerebellar Degeneration, Hemolytic Anemia, and Glutathione DeficiencyArchives of internal medicine (1960), 1974
- Glutathione Synthetase Deficiency, an Inborn Error of Metabolism Involving the γ-Glutamyl Cycle in Patients with 5-Oxoprolinuria (Pyroglutamic Aciduria)Proceedings of the National Academy of Sciences, 1974
- Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissuesAnalytical Biochemistry, 1969
- A COLORIMETRIC MICRO-METHOD FOR THE DETERMINATION OF GLUTATHIONEBiochemical Journal, 1965