[Ca2+]i oscillations in single rat glioma cells induced by thrombin through activation of cell surface receptors
- 1 June 1995
- journal article
- research article
- Published by Wolters Kluwer Health in NeuroReport
- Vol. 6 (9) , 1249-1252
- https://doi.org/10.1097/00001756-199506090-00005
Abstract
Thrombin at nanomolar concentrations induces rapid changes in the second messenger Ca2+ in a glial astrocytetype cell line. Continuous application of the protease thrombin causes regular [Ca2+]i oscillations (amplitude 109 nM, spike length 48 s) which are suppressed by hirudin. Reduction of [Ca2+]ex (from 1.8 mM to 50 μM) reversibly abolishes the oscillations indicating the contribution of Ca2+ influx to generation of the oscillations. Thrombin receptor-activating peptide (TRAP, 1-10 μM) causes similar Ca2+ oscillations which depend, like the oscillations induced by thrombin, on the continuous presence of agonist. Thus, we can deduce that cell surface receptors are responsible for the effect of thrombin on glioma cells.Keywords
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