A method for parallel determination of choline acetyltransferase and muscarinic cholinergic receptors: Application in aged-rat brain
- 1 October 1988
- journal article
- research article
- Published by Springer Nature in Neurochemical Research
- Vol. 13 (10) , 951-955
- https://doi.org/10.1007/bf00970767
Abstract
We have devised a method for the parallel determination of choline acetyltransferase (CAT) and muscarinic cholinergic receptor (mCh-R) in the same brain tissue. The method for CAT activity determination is more rapid, simplified, stable, and economical than the usual Fonnum's method. With our method, age-associated changes in CAT activity and mCh-R levels were examined. Although CAT activity hardly changed with age except in a few areas, mCh-R binding of aged-rats was markedly reduced in all areas. These results suggest that the change in mCh-R represents an age-associated biochemical change in the brain and that determination of CAT activity is not sufficient for the study of age-associated changes in the brain cholinergic system.Keywords
This publication has 22 references indexed in Scilit:
- The Cholinergic Hypothesis of Geriatric Memory DysfunctionScience, 1982
- Pre- and postsynaptic neurochemical alterations in Alzheimer's diseaseBrain Research, 1978
- NECROPSY EVIDENCE OF CENTRAL CHOLINERGIC DEFICITS IN SENILE DEMENTIAThe Lancet, 1977
- SELECTIVE LOSS OF CENTRAL CHOLINERGIC NEURONS IN ALZHEIMER'S DISEASEPublished by Elsevier ,1976
- NEUROTRANSMITTER-RELATED ENZYMES AND INDICES OF HYPOXIA IN SENILE DEMENTIA AND OTHER ABIOTROPHIESBrain, 1976
- Neurotransmitter Receptors in the Brain: Biochemical IdentificationAnnual Review of Physiology, 1976
- A rapid radiochemical method for the determination of choline acetyltransferaseJournal of Neurochemistry, 1975
- Memory Enhancement by Anticholinesterase as a Function of Initial LearningNature, 1967
- REGIONAL STUDIES OF CATECHOLAMINES IN THE RAT BRAIN‐IJournal of Neurochemistry, 1966
- Anticholinesterase-Induced Amnesia and Its Temporal AspectsScience, 1966