LOCATION OF ENZYMES IN AZOTOBACTER AGILIS

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Abstract
Robrish, Stanley A. (University of California, Davis) and Allen G. Marr . Location of enzymes in Azotobacter agilis . J. Bacteriol. 83: 158–168. 1962.—If the cells of Azotobacter agilis are disrupted by osmotic shock, respiratory enzymes and the compounds characteristic of cell wall and cytoplasmic membrane are recovered almost completely in large particles. The large particles obtained by osmotic shock were found by electron microscopy to consist of cell wall, cell membrane, and an internal membrane appearing as either vesicles or tubules in section. These envelopes are free of all the soluble cytoplasmic material and are essentially free of ribosomes. Small particles obtained by osmotic shock are ribosomes; small particles obtained by sonic oscillation consist of both ribosomes and amorphous material, presumably fragments of the envelope.