SYNTHESIS OF FACTOR VIII ANTIGEN BY CULTURED HUMAN ENDOTHELIAL CELLS*
- 1 January 1975
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 240 (1) , 62-69
- https://doi.org/10.1111/j.1749-6632.1975.tb53322.x
Abstract
Endothelial cells have been isolated from freshly obtained human umbilical cords by collagenase digestion of the interior of the umbilical vein and grown in tissue culture. These cells have been identified by morphologic and immunologic criteria. It has been demonstrated that these cultured human endothelial cells synthesize and release factor VIII antigen but not factor VIII clot-promoting activity.Keywords
This publication has 16 references indexed in Scilit:
- Synthesis of Antihemophilic Factor Antigen by Cultured Human Endothelial CellsJournal of Clinical Investigation, 1973
- Culture of Human Endothelial Cells Derived from Umbilical Veins. IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGIC CRITERIAJournal of Clinical Investigation, 1973
- Antihemophilic Factor Antigen. LOCALIZATION IN ENDOTHELIAL CELLS BY IMMUNOFLUORESCENT MICROSCOPYJournal of Clinical Investigation, 1973
- Quantitative Assay of a Plasma Factor Deficient in von Willebrand's Disease that is Necessary for Platelet Aggregation. RELATIONSHIP TO FACTOR VIII PROCOAGULANT ACTIVITY AND ANTIGEN CONTENTJournal of Clinical Investigation, 1973
- Factor VIII on the Vascular Intima: Possible Importance in Haemostasis and ThrombosisNature New Biology, 1973
- Studies on the purification and characterization of human factor VIIIJournal of Clinical Investigation, 1972
- Molecular Forms of Antihaemophilic Globulin in Plasma, Cryoprecipitate and after Thrombin ActivationBritish Journal of Haematology, 1970
- Kidney antihemophilic factor. Partial purification and some propertiesBiochemistry, 1968
- Antihemophilic Factor Release by Perfused Liver and Spleen: Relationship to HemophiliaScience, 1967
- Ethionine-Induced Depression of Plasma Antihemophilic Globulin in the RatExperimental Biology and Medicine, 1954