MODIFICATION OF PH OF NORMAL AND MALIGNANT MOUSE-TISSUE BY HYDRALAZINE AND GLUCOSE, WITH AND WITHOUT BREATHING OF 5-PERCENT CO2 AND 95-PERCENT AIR

  • 15 March 1988
    • journal article
    • research article
    • Vol. 48  (6) , 1543-1547
Abstract
We investigated the effects of hydralazine and glucose on the pH of several nornal tissues and on RIF-1 radiation induced fibrosarcomas of mice that were breathing either air or a mixture of 5% CO2 and 95% air. Our goal was to investigate techniques to maximize the pH differences between tissue and tumors. Hydralazine (10 mg/kg) had only minor effects on pH of tumors and muscle; it lowered liver and kidney pH. In animals additionally breathing the air-gas mixture, pH was further lowered in liver and kidney. Glucose (0.6 mg/kg) by itself caused a major reduction of pH of liver, kidney, and adipose tissue. Only minor effects were seen in tumors and muscle. Causing the animals to breathe the gas-air mixture 4 h after glucose injection partially reversed the glucose effect in all normal tissues but caused additional reductions in tumors, particularly in small (<0.7 cm3) lesions. Thus, this last combination led to maximum differential between the pH of normal tissue and of tumor.