SEPARATION OF 2 FORMS OF HELA DNA POLYMERASE-ALPHA WITH DIFFERENT BINDING AFFINITY TO DNA
- 1 January 1978
- journal article
- research article
- Vol. 69 (2) , 207-212
Abstract
Two forms of DNA polymerase .alpha. were separated from HeLa human cervical cancer cells by DEAE-cellulose column chromatography and affinity chromatography using a DNA-cellulose column. They were eluted from a DEAE-cellulose column at 0.22 M KCl (P-I) and 0.24 M KCl (P-II). On cell fractionation between cytosol and nuclei, P-I was recovered from nuclei and P-II from cytosol. P-I had a higher binding affinity to DNA than P-II by DNA-cellulose column chromatography.This publication has 4 references indexed in Scilit:
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