Isolation of the RAD18 gene of Saccharomyces cerevisiae and construction of rad18 deletion mutants
- 1 February 1989
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 215 (3) , 425-430
- https://doi.org/10.1007/bf00427039
Abstract
The RAD18 gene of Saccharomyces cerevisiae is involved in mutagenic DNA repair. We describe its isolation from a yeast library introduced into the centromeric YCp50 vector, a low copy number plasmid. The insert was sublconed into YCp50 and into the multicopy YRp7 plasmid. RAD18 is not toxic when present in multiple copies but the UV survival response indicates an heterogeneity in the cell population, a fraction of it being more sensitive. A DNA segment, close to RAD18, is toxic on the multicopy plasmid and may correspond to the tRAN sup61 known to be tightly linked to RAD18. Chromosomal deletions of RAD18 were constructed. The gene is not essential and the deleted strains have the properties of single site mutants. Thus, RAD18 appears to be essentially involved in DNA repair metabolism.This publication has 41 references indexed in Scilit:
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