Posttranslational Heterogeneity of Bone Alkaline Phosphatase in Metabolic Bone Disease
- 1 January 1994
- journal article
- research article
- Published by Walter de Gruyter GmbH in cclm
- Vol. 32 (9) , 675-680
- https://doi.org/10.1515/cclm.1994.32.9.675
Abstract
Bone alkaline phosphatase is a marker of osteoblast activity. In order to study the posttranscriptional modification (glycosylation) of bone alkaline phosphatase in bone disease, we investigated the relationship between mass and catalytic activity of bone alkaline phosphatase in patients with osteoporosis and hyperthyroidism. Serum bone alkaline phosphatase activity was measured after lectin precipitation using the Iso-ALP test kit. Mass concentration of bone alkaline phosphatase was determined with an immunoradiometric assay (Tandem-R Ostase). In general, serum bone alkaline phosphatase mass and activity concentration correlated well. The activity : mass ratio of bone alkaline phosphatase was low in hyperthyroidism. Activation energy of the reaction catalysed by bone alkaline phosphatase was high in osteoporosis and in hyperthyroidism. Experiments with neuraminidase digestion further demonstrated that the thermodynamic heterogeneity of bone alkaline phosphatase can be explained by a different glycosylation of the enzyme.Keywords
This publication has 11 references indexed in Scilit:
- Differences between the Sugar Moieties of Liver- and Bone-Type Alkaline Phosphatases: A Re-EvaluationAnnals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1994
- Multiple Forms of Human Serum Alkaline Phosphatase: Detection and QuantitationAnnals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1993
- Glycation of human tissue and serum creatine kinaseClinica Chimica Acta; International Journal of Clinical Chemistry, 1992
- Differences between human liver- and bone-type alkaline phosphatasesClinica Chimica Acta; International Journal of Clinical Chemistry, 1989
- Concurrent Assays of Circulating Bone Gla-Protein and Bone Alkaline Phosphatase: Effects of Sex, Age, and Metabolic Bone Disease*Journal of Clinical Endocrinology & Metabolism, 1988
- Multiple forms of acid and alkaline phosphatases: Genetics, expression and tissue-specific modificationClinica Chimica Acta; International Journal of Clinical Chemistry, 1986
- Phenotypes of placental‐type alkaline phosphatase in seminoma sera as defined by monoclonal antibodiesInternational Journal of Cancer, 1986
- Affinity electrophoresis of human serum alkaline phosphatase isoenzymes in agarose gel containing lectinClinica Chimica Acta; International Journal of Clinical Chemistry, 1986
- Modification of Alkaline Phosphatases by Treatment with GlycosidasesEnzyme, 1985
- Improved electrophoretic resolution of bone and liver alkaline phosphatases resulting from partial digestion with neuraminidaseClinica Chimica Acta; International Journal of Clinical Chemistry, 1984