Studies on the growth ofCladophora glomeratain laboratory continuous-flow culture

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Abstract
Cladophora glomerata grown in continuous-flow culture was found to have optimal specific growth rate (μ) at, or near, 20°C. Specific growth rate increased linearly with increased duration of illumination per day up to 24h, and increased light intensity up to 6000 lx. Undissociated ammoniacal nitrogen (0·185 mg 1-1) reduced μ to 50% of that at 0·010 mg 1-1: 0·077–1·057 mg NO2-N1-1 and 7·2–15·2 mg NO3-N1-1 had no significant effect on μ. At 4·9 mg PO4-P1-1, μ was 48% of that at 1·9 mg1-1. The critical medium PO4-P concentration was less than 0·098 mg1-1. Specific growth rate was reduced to 50% of that in the based medium by 0·036 mg Cu1-1, 0·070 mg Zn1-1 and 1·03 mg Pb1-1. Results are discussed in the context of the natural distribution of the alga in the field situation.

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