Suppression of human polymorphonuclear leukocyte phagocytosis by adenosine analogs
- 1 September 1987
- journal article
- research article
- Published by Springer Nature in Inflammation
- Vol. 11 (3) , 365-369
- https://doi.org/10.1007/bf00915840
Abstract
The effects of adenosine analogs on human polymorphonuclear leukocyte (PMN) phagocytosis to latex beads or sheep red blood cells were investigated in an in vitro experiment. The purine compounds such asN6-phenylisopropyladenosine (PIA), 5′-N-ethylcarboxamido-adenosine (NECA), an A2 adenosine receptor agonist, and adenosine as high as 2 mM had no effect on PMN phagocytosis. In contrast, 2′,5′-dideoxyadenosine (DDA), a P-site adenosine receptor agonist, and 5′-methylthioadenosine (MTA), a naturally occuring purine nucleoside, caused profound inhibition of phagocytic function of PMNs in a dose-dependent manner. Dipyridamole, which blocks purine nucleoside uptake, reversed the suppression due to MTA. Theophylline, an R-site receptor antagonist did not prevent the effect of MTA. These results suggested that phagocytosis of PMNs is suppressed by stimulation of the P-site adenosine receptors.Keywords
This publication has 17 references indexed in Scilit:
- Adenosine Receptors on Human Inflammatory CellsInternational Archives of Allergy and Immunology, 1985
- 2-Chloroadenosine inhibits complement-induced reactive oxygen metabolite production and recovery of human polymorphonuclear leucocytes attacked by complementBiochemical and Biophysical Research Communications, 1985
- 5′-Deoxy-5′-methylthioadenosine inhibition of rat T lymphocyte phosphodiesterase : Correlation with inhibition of Con A induced proliferationBiochemical and Biophysical Research Communications, 1983
- Adenosine metabolism: Modification by S-adenosylhomocysteine and 5′-methylthioadenosineArchives of Biochemistry and Biophysics, 1982
- Adenosine receptors: targets for future drugsJournal of Medicinal Chemistry, 1982
- Trends in the biochemical pharmacology of 5′-deoxy-5′-methylthioadenosineBiochemical Pharmacology, 1982
- Adenosine receptors in the central nervous system: Relationship to the central actions of methylxanthinesLife Sciences, 1981
- Inactivation of S-Adenosylhomocysteine hydrolase by 5′-deoxy-5′-methylthioadenosineBiochemical and Biophysical Research Communications, 1981
- Subclasses of external adenosine receptors.Proceedings of the National Academy of Sciences, 1980
- Modulation of human neutrophil chemotactic responses by cyclic 3′,5′-guanosine monophosphate and cyclic 3′,5′-adenosine monophosphateMetabolism, 1975