Intravascular naked DNA vaccine encoding glycoprotein B induces protective humoral and cellular immunity against herpes simplex virus type 1 infection in mice
- 3 November 2003
- journal article
- Published by Springer Nature in Gene Therapy
- Vol. 10 (25) , 2059-2066
- https://doi.org/10.1038/sj.gt.3302114
Abstract
Naked plasmid DNA (pDNA) vaccine expressing herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) was tested for protective activity against acute HSV-1 infection in mice. The pDNA was intravenously injected into Balb/c mice via their tail vein under high pressure, and the vaccination was performed two times at an interval of 7 days. The gB gene vaccination significantly protected the mice from subsequent intraperitoneal challenge with a lethal dose of HSV-1, which killed all the animals given control plasmid or saline. The protective activity was correlated with the dose of the plasmid inoculated, the survival rate reaching 83% in mice vaccinated with 5 g of pDNA. The vaccinated mice were also protected from latent HSV infection. The immunized mice showed significant elevation in neutralizing antibody against HSV-1 as well as serum levels of interleukin-12 (IL-12) and interferon- (IFN-). When mice were immunized with 5 g of an Epstein–Barr virus (EBV)-based plasmid vector harboring the gB, the cytotoxic T lymphocytes (CTLs) activity and proliferative response for HSV-1 were also induced. The results strongly suggest that intravenous immunization of naked pDNA may induce humoral and cellular immune responses against the virus, leading to a significant prophylactic outcome against HSV-1 infection in mice.Keywords
This publication has 69 references indexed in Scilit:
- Characterization of B virus glycoprotein antibodies induced by DNA immunizationArchiv für die gesamte Virusforschung, 2002
- Epstein–Barr Virus/Human Vector Provides High-Level, Long-Term Expression of α1-Antitrypsin in MiceMolecular Therapy, 2001
- Linear DNAs Concatemerize in Vivo and Result in Sustained Transgene Expression in Mouse LiverMolecular Therapy, 2001
- Direct intra-cardiomuscular transfer of β2-adrenergic receptor gene augments cardiac output in cardiomyopathic hamstersGene Therapy, 2000
- Targeted killing of carcinoembryonic antigen (CEA)-producing cholangiocarcinoma cells by polyamidoamine dendrimer-mediated transfer of an Epstein-Barr virus (EBV)-based plasmid vector carrying the CEA promoterCancer Gene Therapy, 2000
- Effective suicide gene therapy in vivo by EBV-based plasmid vector coupled with polyamidoamine dendrimerGene Therapy, 2000
- DNA-Mediated Immunization in Mice Induces a Potent MHC Class I-Restricted Cytotoxic T Lymphocyte Response to the Hepatitis B Envelope ProteinHuman Gene Therapy, 1995
- Heterologous Protection Against Influenza by Injection of DNA Encoding a Viral ProteinScience, 1993
- Efficient selection for high-expression transfectants with a novel eukaryotic vectorGene, 1991
- Stable replication of plasmids derived from Epstein–Barr virus in various mammalian cellsNature, 1985