BLOOD-CLOTTING TIME IN RABBITS AND ITS VARIATIONS DETERMINED WITH A SIMPLE CAPILLARY METHOD

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Abstract
A simple modification of the capillary method which excludes the disadvantages of the customary method for the detn. of the clotting time is descr. Capillary tubes of 1.25-1.5 mm. diam. and 30 cm. length were connected to 22 gauge hypodermic needles by sealing the capillaries in the center of the connecting cone with paraffin. Multiple blood samples can be rapidly obtained with this equipment by direct veni-puncture. Uncontrolled injury and contact with tissue fluid and the skin surface are thereby minimized. After adequate training, rabbits yield easily reproducible results with this method. 298 samples were obtained from a group of 12 animals, and the normal clotting time was 200 [plus or minus] 2 sec. The mean values for the individual animals were between 180 and 220 sec. with standard errors of the mean from 3-7 sec. Pain or excitement drops the clotting time to values which are essentially different from normal clotting times. The statistical analysis shows that there is a significant diurnal cycle in the variation of clotting times with a low around midnight (185 sec.) and a high in the late afternoon (214 sec). Animals fully adapted to +5[degree]C or + 25[degree]C. do not show a significant difference in normal clotting times.
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