Influence of thyroid status on the membranes of rat liver mitochondria Unique localization of L‐glycerol‐3‐phosphate dehydrogenase

Abstract
The effect of thyroid status on the physical properties of rat liver mitochondrial membranes and on the lipid microenvironment of proteins was investigated. The steady‐state fluorescence anisotropy of diphenyl‐1,3,5‐triene and 1‐[4‐{trimethylaminophenyl} phenyl]‐6‐phenylhexa‐1,3,5‐triene revealed an increase of the order of the membranes with the increase of hormone level. Protein arrangement in the inner mitochondrial membrane altered with the thyroid status, which was reflected by digitonin subfractionation of mitochondria. The microenvironment of FAD‐linked L‐glycerol‐3‐phosphate dehydrogenase was dramatically influenced by thyroxine.

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