A Procedure for the Determination of High-Density Lipoprotein Choline-Containing Phospholipids

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Abstract
A procedure for the determination of [human] serum high-density lipoprotein choline-containing phospholipids is described. The choline-containing phospholipids represent 91-97% of the total serum and high-density lipoprotein phospholipids which were previously determined in relation to liver disease, neoplastic disorders, diabetes mellitus and atherosclerosis. The enzymatic assay is quick, simple and precise. No interference was found from the precipitating agents used to isolate the high-density lipoprotein fraction. Several serum samples were assayed for high-density lipoprotein cholesterol and choline-containing phospholipids. There appears to be a correlation between these 2 lipid classes.

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