NUTRITION STUDIES OF TWO VARIANTS OF LACTOBACILLUS GAYONI

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Abstract
Comparative nutrition studies of 2 interconvertible variants of L. gayoni 8289 were undertaken. The more fastidious variant, strain 45, which requires nucleotides and high amts. of folic acid for rapid growth, can practically dispense with nucleotides by growing in a basal medium of pH 5-5.4 in the presence of large amts. of inorganic phosphate. Ribose-5-phosphate and 1-methyl ribose-3-phosphate are unable to replace mononucleotides. Sorbitan monooleate replaces biotin for strain 45. In addition, it enhances the utilization of phosphate or nucleotides. Under suitable conditions nucleotide-free liver prepns. are also stimulatory. The less fastidious variant, strain 49, is not stimulated by folic acid or nucleotides under usual exptl. conditions and gives only a slight, transitory response to sorbitan monooleate, phosphate, or liver prepns. It is suggested that the respective abilities to synthesize folic acid and nucleotides are interrelated properties of L. gayoni 8289, and that nucleotides may be synthesized near the surface of the cell.