Purification and characterization of recombinant single-chain urokinase produced in Escherichia coli
- 15 July 1986
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 25 (14) , 4041-4045
- https://doi.org/10.1021/bi00362a008
Abstract
Recombinant single-chain urokinase (rUK1) has been purified from Escherichia coli. The purification utilizes a refractile body purification, followed by batch DE-52 cellulose extraction, hydroxylapatite chromatography, and S-200 chromatography. Two-chain rUK (rUK2) is separated from rUK1 on benzamidine-Sepharose. The purification proteases early in the procedure so the rUK1 will not be cleaved to rUK2. The rUK1 has been characterized by amino-terminal analysis as well as carboxy-terminal analysis after cleavage by plasmin.This publication has 18 references indexed in Scilit:
- Effective and fibrin-specific clot lysis by a zymogen precursor form of urokinase (pro-urokinase). A study in vitro and in two animal species.Journal of Clinical Investigation, 1984
- Purification of human fibroblast urokinase proenzyme and analysis of its regulation by proteases and protease nexin.Journal of Biological Chemistry, 1984
- Purification and partial characterization of a single-chain high-molecular-weight form of urokinase from human urineArchives of Biochemistry and Biophysics, 1983
- Purification of zymogen to plasminogen activator from human glioblastoma cells by affinity chromatography with monoclonal antibodyBiochemistry, 1982
- A proenzyme form of human urokinase.Journal of Biological Chemistry, 1982
- The Complete Amino Acid Sequence of Low Molecular Mass Urokinase from Human UrineHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1982
- Assay of urokinase activity in plasma with a chromogenic substrateThrombosis Research, 1981
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- A study of proteases and protease-inhibitor complexes in biological fluidsThe Journal of Experimental Medicine, 1978
- Urokinase an activator of plasminogen from human urine I. Isolation and propertiesBiochimica et Biophysica Acta, 1957