Comparison of the Column Performance of Narrow-Bore and Standard-Bore Columns for the Chromatographic Determination of -, -, -, and -Tocopherol
Open Access
- 1 January 2001
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 39 (1) , 1-6
- https://doi.org/10.1093/chromsci/39.1.1
Abstract
A comparison of the performance of narrow-bore (2.1-mm i.d.) and standard-bore (4.6-mm i.d.) analytical silica columns having the same length is completed for the resolution of α-, β-, γ-, and δ-tocopherol. The studies are performed on high-performance liquid chromatographic equipment with minimum extracolumn contribution. Column permeabilities are 1.16 × 10−9 and 2.48 × 10-9 cm2 for narrow and standard bore, respectively. The narrow-bore column gives up to a 7 times increase in sensitivity compared with a standard-bore column at equivalent running times for the analytes. Approximately one-third solvent savings can be achieved with the narrow-bore column. Theoretical plates of the standard-bore column are higher than that of the narrow-bore column.Keywords
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