Determination of the Complete Amino Acid Sequence of Recombinant Human γ-Interferon Produced inEscherichia coli
- 1 August 1986
- journal article
- research article
- Published by Mary Ann Liebert Inc in Journal of Interferon Research
- Vol. 6 (4) , 331-336
- https://doi.org/10.1089/jir.1986.6.331
Abstract
The complete amino acid sequence of recombinant human γ-interferon (HuIFN-γ) produced in Escherichia coli was determined using a gas-phase protein sequencer. The sequence was established by automated Edman degradation on the intact protein and its peptides obtained after Staphylococcus aureus V8 protease or trypsin digestion. The result was identical to the amino acid sequence predicted from the nucleotide sequence of the cloned HuIFN-γ cDNA except that it was missing the four carboxy-terminal residues, Arg-Ala-Ser-Gln.Keywords
This publication has 9 references indexed in Scilit:
- Utility of the gas-phase sequencer for both liquid- and solid-phase degradation of proteins and peptides at low picomole levelsAnalytical Biochemistry, 1984
- Natural human interferon-gamma. Complete amino acid sequence and determination of sites of glycosylation.Journal of Biological Chemistry, 1984
- Effects of glycosidase treatment on the physicochemical properties and biological activity of human interferon-gamma.Journal of Biological Chemistry, 1983
- Expression of human immune interferon cDNA in E. coli and monkey cellsNature, 1982
- Molecular Weight of Human Gamma Interferon Is Similar to that of Other Human InterferonsScience, 1982
- Amino acid sequence of a human leukocyte interferon.Proceedings of the National Academy of Sciences, 1981
- A gas-liquid solid phase peptide and protein sequenator.Journal of Biological Chemistry, 1981
- A Protein SequenatorEuropean Journal of Biochemistry, 1967
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951