Demonstration that the BchH protein of Rhodobacter capsulatus activates S‐adenosyl‐l‐methionine:magnesium protoporphyrin IX methyltransferase
- 5 May 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 407 (3) , 337-342
- https://doi.org/10.1016/s0014-5793(97)00371-2
Abstract
The bchH gene of Rhodobacter capsulatus has been cloned into an expression strain of Escherichia coli. Following induction of expression of the BchH protein, it was found that the E. coli strain also accumulated porphyrins with the fluorescence properties of protoporphyrin and zinc protoporphyrin. It was also found that the soluble BchH protein increased the activity of S‐adenosyl‐l‐methionine:magnesium protoporphyrin IX methyltransferase, when mixed with membranes of an expression strain of E. coli into which the bchM gene (which encodes the methyltransferase) had been cloned, as well as membranes of a bchH mutant of R. capsulatus.Keywords
This publication has 36 references indexed in Scilit:
- A Putative Mg Chelatase Subunit from Arabidopsis thaliana cv C24 (Sequence and Transcript Analysis of the Gene, Import of the Protein into Chloroplasts, and in Situ Localization of the Transcript and ProteinPlant Physiology, 1996
- The bacteriochlorophyll biosynthesis gene, bchM, of Rhodobacter sphaeroides encodes S‐adenosyl‐L‐methionine: Mg protoporphyrin IX methyltransferaseFEBS Letters, 1994
- Directed Mutational Analysis of Bacteriochlorophyll a Biosynthesis in Rhodobacter capsulatusJournal of Molecular Biology, 1994
- Regulation of carotenoid and bacteriochlorophyll biosynthesis genes and identification of an evolutionarily conserved gene required for bacteriochlorophyll accumulationJournal of General Microbiology, 1993
- An overlap between operons involved in carotenoid and bacteriochlorophyll biosynthesis inRhodobacter capsulatusFEMS Microbiology Letters, 1992
- Mapping and sequencing of an actively transcribedEuglena gracilis chloroplast gene (ccsA) homologous to theArabidopsis thaliana nuclear genecs(ch‐42)FEBS Letters, 1992
- Nucleotide Sequence of S-Adenosyl-l-Methionine: Magnesium Protoporphyrin Methyltransferase from Rhodobacter capsulatusPlant Physiology, 1992
- Chloroplast biogenesis: detection of divinylprotochlorophyllide ester in higher plantsBiochemistry, 1980
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970