Effect of salinity on the osmoregulatory cells in the tracheal gills of the stonefly nymph, Paragnetina media (Plecoptera: Perlidae)

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Abstract
The ultrastructure of the osmoregulatory cells in the integument of the stonefly nymph, Paragnetina media, shows changes in media hypotonic, isotonic, and hypertonic to the hemolymph. Exposure to hypotonic concentration results in an increase in the plasma membrane folds, mitochondria, and microtubules in the cytoplasm. In isotonic and hypertonic concentrations, the cells show cellular disorganization and apparent loss in their function. These results suggest that the structural changes in the osmoregulatory cells are related to the osmotic condition of the medium, and these cells probably act as extrarenal sites for the absorption of ions from the hypoosmotic medium.

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