Homogeneous time-resolved fluoroimmunoassay of thyroxin in serum.
Open Access
- 1 November 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 34 (11) , 2320-2322
- https://doi.org/10.1093/clinchem/34.11.2320
Abstract
We describe a rapid, simple nonseparation fluoroimmunoassay for determination of thyroxin in serum. The assay is based on the labeling of thyroxin directly with a fluorescent europium chelate, the fluorescence of which is quenched on binding to an antithyroxin antibody. With the assay buffer we used, maximum quenching is 90%. The rapid achievement of equilibrium in the assay solution, regardless of the sequence of reagent additions, allows fast measurement of thyroxin. Precision was good (CV less than 5%) within the clinical range for total thyroxin (50-300 nmol/L), and results correlated well with those by a commercial radioimmunoassay.This publication has 1 reference indexed in Scilit:
- Europium as a label in time-resolved immunofluorometric assaysAnalytical Biochemistry, 1984