Codetection of a Mixed Population ofcandHPV62 Containing Wild‐Type and Disrupted E1 Open‐Reading Frame in a 45‐Year‐Old Woman with Normal Cytology
Open Access
- 1 October 2004
- journal article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 190 (7) , 1303-1309
- https://doi.org/10.1086/423855
Abstract
We have cloned, sequenced, and characterized the complete genome of a novel human papillomavirus (HPV), candHPV62. During cloning, 2 candHPV62 viral isolates were recovered from a single cervical sample; 1 had all anticipated HPV open-reading frames (ORFs) intact, whereas the other exhibited an E1 frame-shift mutation. Further experiments indicated that the 2 strains were equivalent in abundance. It appears that an early mutation occurred within the E1 ORF, which was transcomplemented by an intact E1 protein. A search of the HPV database identified disruption of the E1 ORF in the cloned reference isolates of HPV16, HPV53, HPV56, and HPV72. These data suggest that disruption of the E1 ORF in genital HPVs is not uncommon.Keywords
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