Biogenesis of Multiple Cellulase Components of Pseudomonas fluorescens var. cellulosa

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Abstract
In the culture medium of a cellulolytic bacterium, Pseudomonas fluorescens var. cellulosa, several extracellular cellulase [EC 3.2.1.4] components of different molecular weights were found, and the number of components increased in parallel with the culture period. Of these cellulase components, at least five (peaks I, II, III, IV, and V) were electrophoretically separated further into two components, A and B, in varying proportions. Peak I and peak V were composed almost exclusively of B and A, respectively. The ratio of total A to total B increased during bacterial growth. Moreover, peak I which had the largest molecular weight among the detectable extracellular cellulases was found to decrease in amount during culture, and almost disappeared at the later stages of culture. On the other hand, only peak I was found in the intrawall fraction during earlier stages of culture in which active secretion of extracellular cellulases was taking place. These facts strongly suggest that the multiple cellulase components in the culture medium are secondarily formed from peak I outside the cell.

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