Growth and Secondary Product Formation of in vitro Cultures from the Liverwort Reboulia hemisphaerica

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Abstract
Reboulia hemisphaerica has been cultivated on Gamborg B5 medium with various sugars. Differentiated cultures grew best on agar medium with the addition of 0.5% sucrose and a light regime of 18 h light/6h dark . Callus was induced either by a combination of phytohor­mones (2 mg/l α-naphthylacetic acid + 1 mg/l kinetin) or by 4% glucose. The differentiated cultures on agar produced sesquiterpenes in a tenfold increased amount compared to undiffer­ entiated suspension cultures. Cultures kept in da rk produced no sesquiterpenes, but 5-hydroxy-7,8,4′-trimethoxyflavone was present whereas apigenin-7,4′-dimethylether was the only flavonoid in cultures cultivated in the light.

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