Sequential expression of CD34 and CD33 antigens on myeloid colony‐forming cells
- 1 February 1989
- journal article
- research article
- Published by Wiley in European Journal of Haematology
- Vol. 42 (2) , 143-149
- https://doi.org/10.1111/j.1600-0609.1989.tb01203.x
Abstract
The expression of CD33 and CD34 antigens on human bone marrow cells was examined by fluorescence‐activated cell sorting and colony assays. A marked difference of antigen expression was observed on sorted progenitors of the granulocyte/macrophage lineage (CFU‐GM) with respect to enrichment and maturation status. Single‐color cell sorting revealed no difference of enrichment by anti‐CD33 antibody between day‐7 and d‐14 progenitors, while anti‐CD34 antibody preferentially enriched d‐14 colony‐forming units (CFU). By two‐color cell sorting it could be shown that enrichment of d‐14 CFU‐GM occurred mostly in the CD34+CD33‐ and to a lesser extent in the double‐positive fraction. In contrast, there was no difference in degree of enrichment between d‐7 and d‐14 CD34‐CD33+ CFU‐GM. From these data we conclude that, during early myelopoiesis, CD34 antigens are gradually lost while CD33 antigens are acquired.Keywords
This publication has 13 references indexed in Scilit:
- HLA-class II antigens on human hematopoietic progenitorsAnnals of Hematology, 1987
- Cell surface antigens on human marrow cells: Dissection of hematopoietic development using monoclonal antibodies and multiparameter flow cytometryThe International Journal of Cell Cloning, 1987
- Subpopulations of common acute lymphoblastic leukemia antigen-positive lymphoid cells in normal bone marrow identified by hematopoietic differentiation antigensBlood, 1986
- Monoclonal antibody 12-8 recognizes a 115-kd molecule present on both unipotent and multipotent hematopoietic colony-forming cells and their precursorsBlood, 1986
- Co-ordinate expression of BI.3C5 and HLA-DR antigens on haemopoietic progenitors from chronic myeloid leukaemiaLeukemia Research, 1986
- Identification of a membrane glycoprotein associated with haemopoietic progenitor cellsLeukemia Research, 1985
- A novel monoclonal antibody BI-3C5 recognises myeloblasts and non-B non-T lymphoblasts in acute leukaemias and CGL blast crises, and reacts with immature cells in normal bone marrowLeukemia Research, 1985
- Enrichment of pluripotent hemopoietic progenitor cells from human bone marrowBlood, 1984
- Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells.The Journal of Immunology, 1984
- A monoclonal antibody reactive with normal and leukemic human myeloid progenitor cellsLeukemia Research, 1984