Hydroxylation of Chlorinated and Unchlorinated Chlorophylls in vitro

Abstract
Chlorophyll a is hydroxylated quantitatively in the 132-position, when chromatographed on silica gel thin-layer plates. This was shown by using HPLC as a non-destructive method für analyzing photosynthetic pigments. The hydroxy-group of Chl RC I, a 132-hydroxy-20-chloro-chl a. described by Dörnemann and Senger (1986) and Scheer et al. (1986), is shown to be artificially introduced during the purification procedure by TLC.