Limitations of commonly used spectrophotometric assay methods for phosphoenolypyruvate carboxykinase activity in crude extracts of muscle
- 14 July 1982
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 206 (1) , 147-152
- https://doi.org/10.1042/bj2060147
Abstract
Phosphoenolpyruvate carboxykinase activity in crude extracts of [rat] muscle has frequently been determined by using a continuous spectrophotometric method, which grossly overestimates enzyme activity. NADH oxidation attributed to phosphoenolpyruvate carboxykinase activity in the assay is due to lactate production. Under the normal assay conditions, Na+ ions stimulate pyruvate kinase, providing pyruvate for lactate formation by lactate dehydrogenase and sufficiently to account for most of the observed NADH oxidation.This publication has 20 references indexed in Scilit:
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