Phenylalanyl-tRNA Synthetase from Baker’s Yeast: Specificity and Quantitation of Affinity Elution with tRNA
- 1 January 1976
- journal article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 357 (1) , 819-824
- https://doi.org/10.1515/bchm2.1976.357.1.819
Abstract
TRNAPhe is able to elute phenylalanyl-tRNA synthetase from cation exchangers in a 1:1 ratio. Elution of phenylalanyl-tRNA synthetase in a 1:1 ratio is also observed for four noncognate tRNAs investigated, specific for valine, serine, isoleucine and tyrosine. If protein mixtures are subjected to affinity elution the cognate pair [tRNAPhe-phenylalanyl-tRNA synthetase] is eluted first, followed by noncognate pairs. The unspecific elution is not influenced by complexation of phenylalanyl-tRNA synthetase with an analog of phenylalanyl-adenylate.Keywords
This publication has 3 references indexed in Scilit:
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- Synthese von Inhibitoren für die Phenylalanyl‐tRNA‐Synthetase: Methylen‐Analoge des Phenylalanyl‐adenylatsEuropean Journal of Inorganic Chemistry, 1973
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