Purification and Characterization of 6-Phospho-β-galactosidase fromLactobacillus gasseriJCM 1031
- 1 January 1996
- journal article
- Published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry
- Vol. 60 (1) , 139-141
- https://doi.org/10.1271/bbb.60.139
Abstract
6-Phospho-beta-galactosidase from Lactobacillus gasseri JCM 1031 was purified from lactobacilli to homogeneity, about 118-fold, with 0.5% recovery by several chromatographies. The molecular mass and isoelectric point of the purified enzyme were 58 kDa and pI 5.5, respectively. The Km and Vmax for o-nitrophenyl-beta-D-galactopyranoside-6-phosphate were 0.8 mM and 116.4 mumol/min/mg, respectively. Reducing agent, Fe2+ ion, and EDTA activated but PCMB, Zn2+, and Hg2+ ions strongly inhibited the enzymatic activity.Keywords
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