Oxidation of glutathione by free radical intermediates formed during peroxidase‐catalysed N‐demethylation reactions
- 17 October 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 162 (2) , 411-415
- https://doi.org/10.1016/0014-5793(83)80797-2
Abstract
Horseradish peroxidase-catalyzed N-demethylation of aminopyrine and dimethylaniline results in generation of free radical intermediates which can interact with glutathione (GSH) to form a glutathione radical. This can either dimerize to yield glutathione disulfide or react with O2 to form oxygenated products of glutathione. Ethylmorphine is not a substrate in the peroxidase-mediated reaction, and free radical intermediates which react with GSH, are not formed from aminopyrine and dimethylaniline when the horseradish peroxidase/H2O2 system is replaced by liver microsomes and NADPH. Therefore, it appears unlikely that formation of free radical intermediates can be responsible for the depletion of GSH observed during N-demethylation of several drugs in isolated liver cells.Keywords
This publication has 13 references indexed in Scilit:
- The horseradish peroxidase-catalyzed oxidation of 3,5,3',5'-tetramethylbenzidine. Free radical and charge-transfer complex intermediates.Published by Elsevier ,2021
- Prostaglandin synthetase catalyzed activation of paracetamolBiochemical Pharmacology, 1982
- Metabolism of hydrogen peroxide in isolated hepatocytes: Relative contributions of catalase and glutathione peroxidase in decomposition of endogenously generated H2O2Archives of Biochemistry and Biophysics, 1981
- Radical mechanism of aminopyrine oxidation by cumene hydroperoxide catalyzed by purified liver microsomal cytochrome P-450Archives of Biochemistry and Biophysics, 1980
- [4] Isolation and use of liver cellsPublished by Elsevier ,1978
- A fluorometric method for determination of oxidized and reduced glutathione in tissuesAnalytical Biochemistry, 1976
- Hydroperoxide dependent O-dealkylation reactions catalyzed by liver microsomal cytochrome P450Biochemical and Biophysical Research Communications, 1975
- Oxidation in the NADP system and release of GSSG from hemoglobin‐free perfused rat liver during peroxidatic oxidation of glutathione by hydroperoxidesFEBS Letters, 1972
- The colorimetric estimation of formaldehyde by means of the Hantzsch reactionBiochemical Journal, 1953
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951