Epigenetic inactivation of RASSF1A candidate tumor suppressor gene at 3p21.3 in brain tumors
- 30 October 2003
- journal article
- research article
- Published by Springer Nature in Oncogene
- Vol. 22 (49) , 7862-7865
- https://doi.org/10.1038/sj.onc.1207082
Abstract
The human Ras association domain family 1A (RASSF1A) gene, recently isolated from the lung and breast tumor suppressor locus 3p21.3, is highly methylated in primary lung, breast, nasopharyngeal and other tumors, and re-expression of RASSF1A suppresses the growth of several types of cancer cells. Epigenetic inactivation of RASSF1A by promoter hypermethylation is also important in the development of several human cancers. The methylation status of the promoter region of RASSF1A was analysed in primary brain tumors and glioma cell lines by methylation-specific polymerase chain reaction. In primary brain tumors, 25 of 46 (54.3%) gliomas and five of five (100%) medulloblastomas showed RASSF1A methylation. In benign tumors, only one of 10 (10%) schwannomas and two of 12 (16.7%) meningiomas showed RASSF1A methylation. The RASSF1A promoter region was methylated in all four glioma cell lines. RASSF1A was re-expressed in all methylated cell lines after treatment with the demethylating agent 5-aza-2′-deoxycytidine. Methylation of the promoter CpG islands of the RASSF1A may play an important role in the pathogenesis of glioma and medulloblastoma.Keywords
This publication has 17 references indexed in Scilit:
- Tumor suppressor genes on chromosome 3p involved in the pathogenesis of lung and other cancersOncogene, 2002
- Blockage of Ca2+-permeable AMPA receptors suppresses migration and induces apoptosis in human glioblastoma cellsNature Medicine, 2002
- Aberrant promoter methylation and silencing of the RASSF1A gene in pediatric tumors and cell linesOncogene, 2002
- RASSF1A promoter region CpG island hypermethylation in phaeochromocytomas and neuroblastoma tumoursOncogene, 2001
- High-Resolution Chromosome 3p Allelotyping of Breast Carcinomas and Precursor Lesions Demonstrates Frequent Loss of Heterozygosity and a Discontinuous Pattern of Allele LossThe American Journal of Pathology, 2001
- The candidate tumor suppressor gene, RASSF1A , from human chromosome 3p21.3 is involved in kidney tumorigenesisProceedings of the National Academy of Sciences, 2001
- Malignant glioma: genetics and biology of a grave matterGenes & Development, 2001
- Methylation associated inactivation of RASSF1A from region 3p21.3 in lung, breast and ovarian tumoursOncogene, 2001
- Molecular cytogenetic analysis of medulloblastomas and supratentorial primitive neuroectodermal tumors by using conventional banding, comparative genomic hybridization, and spectral karyotypingJournal of Neurosurgery, 2000
- Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.Proceedings of the National Academy of Sciences, 1996