Identification of human gene complementing ts AlS9 mouse L-cell defect in DNA replication following DNA-mediated gene transfer
- 1 July 1988
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 14 (4) , 371-379
- https://doi.org/10.1007/bf01534645
Abstract
The temperature-sensitive (ts) mouse L-cell, ts AlS9, is defective in a gene required for nuclear DNA replication early in the S phase of the cell cycle. Human DNA sequences were introduced into ts AlS9 cells together with the plasmid pSV2neo, which can confer resistance to the drug geneticin. Cotransformants, expressing both the plasmid-derived neomycin gene and the transferred human AlS9 gene, were selected for growth in the presence of the drug at the nonpermissive temperature (npt). The resulting transformants retained a common set of human-specific Alu repetitive DNA sequences. These are likely to be accommodated within, or in proximity to, the transferred human AlS9 gene. The results obtained provide the basis for cloning human genes required for DNA replication.Keywords
This publication has 37 references indexed in Scilit:
- Isolation of the human gene that complements a temperature-sensitive cell cycle mutation in BHK cells.Molecular and Cellular Biology, 1987
- Cloning of a human S-phase cell cycle gene: use of transient expression for screening.Molecular and Cellular Biology, 1987
- Molecular cloning of a human gene that regulates chromosome condensation and is essential for cell proliferation.Molecular and Cellular Biology, 1986
- DNA-Mediated Gene Transfer in Mammalian Gene CloningPublished by Springer Nature ,1984
- Association between Primase and DNA Polymerase α in Murine CellsPublished by Springer Nature ,1984
- Polyoma and Cell Chromatin Replication Studied in Mouse Cells which Exhibit Temperature-Sensitive DNA Synthesis because they are S ts or G1 tsPublished by Springer Nature ,1983
- DNA-Mediated Transfer of an RNA Polymerase II Gene: Reversion of the Temperature-Sensitive Hamster Cell Cycle Mutant TsAF8 by Mammalian DNAMolecular and Cellular Biology, 1982
- Poly(ADP-ribose) polymerase activity in mouse cells which exhibit temperature-sensitive DNA synthesisBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981
- Temperature-Sensitive Mutants in the Study of Cell Cycle Progression in Mammalian CellsPublished by Elsevier ,1980
- Semi-conservative and non-conservative replication of DNA in temperature-sensitive mouse L-cellsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1977