Use of Hydrophilic Polytetrafluoroethylene(HPTFE) Membrane in Detecting Cryptosporidium Oocysts from Source Water.

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Abstract
A filtration and concentration method using hydrophilic polytetrafluoroethylene (HPTFE) membrane for detecting Cryptosporidium oocysts in source water is described. Ten liters of sample water spiked with approximately 115 or 230 oocysts, were filtered through 142 mm diameter HPTFE membrane with a pore size of 5.0μm. The oocysts captured on the filter were eluted by vortexing then concentrated by centrifugation. Subsequently, the oocysts in the suspension were purified by the immunomagnetic separation (IMS) method and stained with IFA. In addition, recovery efficiencies of the capsule filter filtration method using the IMS method were evaluated. The mean recovery efficiencies obtained were 48% for source water (n=37) and 20% for tap water (n=3) by the HPTFE membrane method, and 19% for source water (n=12) and 5% for tap water by the capsule filter filtration method (n=3) . The total processing (filtration and concentration) time for the HPTFE membrane method was 30 min. These findings demonstrated that the HPTFE method was simple, easy, economic, and rapid, with considerably high recovery efficiency.

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