SENSITIVE AND SPECIFIC IMMUNORADIOMETRIC ASSAYS FOR HUMAN INTERLEUKIN-1-ALPHA

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  • 1 June 1988
    • journal article
    • research article
    • Vol. 7  (2) , 119-127
Abstract
In order to estimate human IL-1.alpha. in physiological samples we have established sensitive and specific immunoradiometric assays using monoclonal and polyclonal antibodies. Solid phase assays employing two different monoclonal antibodies were suitable for estimating rDNA derived IL-17a but were relatively insensitive. Sensitivity was increased by using a monoclonal antibody to capture antigen and 125I-labelled polyclonal sheep antibodies to develop the assay. This assay could detect as little as 20=pg/ml IL-1.alpha. and could be used for assaying antigen in monocyte supernatants, in human skin extracts and in joint fluid from patients with inflammatory disease. It was entirely specific for IL-1.alpha. and was unaffected by non-IL-1.alpha. components present in serum. It is therefore suitable for monitoring IL-1.alpha. concentrations in the circulation of patients undergoing therapy with the monokine.