Evaluating Glass, Polystyrene, and Polypropylene Containers for Semen Collection and Sperm Washing

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Abstract
The effects of glass, polystyrene, and polypropylene containers on semen parameters as measured by Cell Soft computer-assisted semen analysis and hypoosmotic swelling (HOS) tests were assessed in unwashed specimens and again after sperm washing and swim-up procedures. A superiority in unwashed specimens was observed in glass versus polystyrene concerning velocity, motility percentage, and HOS testing (p < 0.01). Also, superiority of glass versus polystyrene was demonstrated for linearity and ALH (p < 0.05). Glass was only superior to polypropylene in categories of motility and HOS testing (p < 0.05 and p < 0.01, respectively). There was no category in which plastic was statistically superior to glass in the unwashed specimens. Concerning washed and swim-up specimens, there were no semen parameters in which there was any superiority demonstrated of either glass or plastic. Unless some future proof that the differences in semen parameters demonstrated in this study have no clinical significance, these data suggest that the collection of semen samples for sperm analysis or therapeutic use should be performed in glass containers.