STUDIES OF FACTORS INVOLVED IN OXIDATIVE PHOSPHORYLATION

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Abstract
Several soluble factors (coupling factors) have been shown to be required for phosphorylation associated with electron transport in submitochondrial particles. Requirement for coupling factor 1 in the Pi32-ATP exchange reaction has been demonstrated after brief exposure of submitochondrial particles to 4 M urea. Such urea-treated particles were devoid of ATPase activity but conferred oligomycin sensitivity on added ATPase. Treatment of submitochondrial particles with trypsin also resulted in a loss of the Pi 32-ATP exchange reaction. Restoration of activity was achieved by the addition of a new factor (F3). Partially purified preparations of this factor contained pyrophosphatase activity, which was stimulated by addition of ATPase. The possible role of the soluble coupling factors was discussed.