SEPARATION BY ETHANOL OF COMMON AND SOMATIC ANTIGENS OF ENTEROBACTERIACEAE

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Abstract
Suzuki , T. (Children's Hospital, Buffalo, N.Y.), E. A. Gorzynski, and E. Neter . Separation by ethanol of common and somatic antigens of Enterobacteriaceae. J. Bacteriol. 88: 1240–1243. 1964.—Ethanol in a concentration of 85% permits separation from crude culture supernatant fluids of the common enterobacterial antigen and the O antigen, the former being ethanol-soluble, the latter being ethanol-insoluble. The evidence was obtained in hemagglutination, hemolysis, and hemagglutination-inhibition tests. In contrast to the crude antigens obtained from enteric bacteria other than Escherichia coli O14, the ethanol-soluble fraction is immunogenic in the rabbit upon intravenous injection, and antibodies against the common antigen are produced in relatively high titers. Aqueous mixtures of ethanol-soluble and -insoluble fractions engender antibodies against the common antigen in titers significantly lower than those stimulated by the soluble fraction alone. Ethanol-treated whole antigen fails to stimulate antibody formation against this antigen. These results suggest the presence of an inhibitor in the ethanol-insoluble fraction.

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