The nodulation of micro-propagated plants ofParasponia andersoniiby tropical legume rhizobia

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Abstract
A simple clonal micro-propagation system for Parasponia andersonii was employed to study the nodulation response of this non-legume to inoculation by the broad host range Rhizobium sp. NGR234, isolated from Lablab purpureus, and also to tropical legume rhizobia isolated from Aeschynomene species. Partially effective nodules, assayed by acetylene reduction and 15N dilution procedures, were induced with strain NGR234 and its spontaneous streptomycinresistant mutant ANU240. Effective nodules were produced by one of the Aeschynomene strains (ORS302) tested, with rates of acetylene reduction comparable to those of root nodules produced by Bradyrhizobium strain CP279, originally isolated from P. andersonii. Light and transmission electron microscopy showed that there was a correlation between the nitrogen fixing capability of the symbiosis between NGR234 and Parasponia and the number of persistent infection (fixation) threads within the nodule cells.

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