1H homonuclear editing of rat brain using semiselective pulses.
- 1 May 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (10) , 3115-3118
- https://doi.org/10.1073/pnas.82.10.3115
Abstract
We have used a semiselective Hahn spin-echo sequence of the form (1331)-tau-(2662)-tau-AQ, delivered by a surface coil to obtain high-resolution 1H NMR spectra from the brains of intact dead rats. This sequence gave suppression of the tissue water resonance by a factor of 80,000 when tau = 68 ms. Delivery of a frequency-selective Dante pulse train to the alpha-CH resonance of lactate at 4.11 ppm, simultaneously with the 2662 refocusing pulse, altered the j-modulation in the spin-coupled beta-CH3 protons. Subtraction of this spectrum from one in which the Dante was ineffective gave an edited spectrum containing only the beta-CH3 resonance of lactate at 1.31 ppm. When the position of the Dante was shifted to 3.78 ppm to selectively invert the alpha-CH protons of alanine, an edited spectrum of alanine was obtained.This publication has 3 references indexed in Scilit:
- Homonuclear 1H double-resonance difference spectroscopy of the rat brain in vivo.Proceedings of the National Academy of Sciences, 1984
- Detection of cerebral lactate in vivo during hypoxemia by 1H NMR at relatively low field strengths (1.9 T).Proceedings of the National Academy of Sciences, 1984
- High-resolution 1H nuclear magnetic resonance study of cerebral hypoxia in vivo.Proceedings of the National Academy of Sciences, 1983