CHROMOSOMAL CHARACTERISTICS AND NONRANDOM DISTRIBUTION OF SISTER CHROMATID EXCHANGES IN LYMPHOBLASTOID CELL LINES ISOLATED FROM ACUTE LEUKEMIAS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 24  (5) , 537-545
Abstract
Serial cytogenetic characteristics of 4 lymphoblastoid cell lines derived from the peripheral blood of 4 patients with acute leukemia are presented, including G and C banding techniques and the analysis of sister chromatid exchanges by the FPG technique. One of these lines became predominantly diploid, although originally pseudodiploid, while the other 3 changed from diploid to heteroploid during prolonged cultivation, with the appearance of a marker chromosome in 1 of them. The mean number of sister chromatid exchanges per mitosis (9.6) did not differ fron the control value obtained in short-term cultivations of lymphocytes of healthy subjects. All lines showed a significantly increased number of sister chromatid exchanges in B group chromosomes. The contribution of the FPG technique for the study of cell kinetics is briefly discussed.

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