Proteome analysis ofSchizosaccharomyces pombeby two‐dimensional gel electrophoresis and mass spectrometry

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Abstract
The fission yeastSchizosaccharomyces pombe(S. pombe) is a unicellular eukaryote and contains many genes and regulatory mechanisms that are close to those of mammals. In this study, we performed a global proteomic analysis of the fission yeastS. pombewild type h−SL 972 proteome. More than 1500 protein spots were visualized on silver stained 2‐D gels in the 3–10 pIrange with a high resolution and high reproducibility. Protein identification was carried out by MALDI‐TOF‐MS and/or nanoLC‐MS/MS. Advantage of the complementarity of these two MS approaches was used to enhance the identification quality. So far, 364 proteins (representing 157 different proteins) have been identified. We report here the identification of 117 new proteins on our 2‐D reference map of this yeast compared to the first reference map. Of these identified proteins, 40.1% were involved in metabolism. The present work provides a very useful tool for all studies relying onS. pombeas a model organism and is a considerable complement to the first reference map ofS. pombepublished recently by Sun and coworkers (Sun, N., Jang, J., Lee, S., Kim, S. et al..,Proteomics2005,5, 1574–1579).