Quantitation of major allergens in dust samples from urban populations collected in different seasons in two climatic areas of the Basque region (Spain)

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Abstract
We present the results of allergen content evaluation in 80 dust samples from 31 homes of atopic patients from two climatic areas (humid and subhumid), collected in two seasons of the year (autumn and winter). Monoclonal antibody-based immunoassays were used to quantify Der p 1, Der f 1, Der 2, Lep d 1, and Pel d 1. The results were compared according to climate, season, and the type of sensitization (Pyroglyphidae mites, storage mites, or grass pollens). We underline the predominance of Dermatophagoides pteronyssinus (89% of samples) over D. farinae (16% of samples) in our environment. Der p 1 rates were higher in the humid area (Mann-Whitney P P P <0.05), whereas this allergen was not detected in the homes of pollen-allergic patients. Pel d 1 was detected in nine of the 31 homes (16% of samples) although there was a cat in only one home.

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