Appearance of Membrane-bound Iron-Sulfur Centers and the Photosystem I Reaction Center during Greening of Barley Leaves
Open Access
- 1 July 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 60 (1) , 76-80
- https://doi.org/10.1104/pp.60.1.76
Abstract
Dark-grown barley (Hordeum vulgare) etioplasts were examined for their content of membrane-bound iron-sulfur centers by electron paramagnetic resonance spectroscopy at 15K. They were found to contain the high potential iron-sulfur center characterized (in the reduced state) by an electron paramagnetic resonance g value of 1.89 (the “Rieske” center) but did not contain any low potential iron-sulfur centers. Per mole of cytochrome f, dark-grown etioplasts and fully developed chloroplasts had the same content of the Rieske center. During greening of etioplasts under continuous light, low potential bound iron-sulfur centers appear. In addition, the photosystem I reaction center, as measured by the photooxidation of P700 at 15K, also became functional; during greening the appearance of a photoreducible low potential iron-sulfur center paralleled the appearance of P700 photoactivity.This publication has 18 references indexed in Scilit:
- Development of the Primary Photochemical Apparatus of Photosynthesis during Greening of Etiolated Bean LeavesPlant Physiology, 1976
- Composition, structure, and function of complex III of the respiratory chainBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1976
- Correlation of reaction-center chlorophyll (P-700) oxidation and bound iron-sulfur protein photoreduction in chloroplast Photosystem I at low temperaturesBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1976
- E.P.R. spectra of iron-sulphur proteins in dimethylsulphoxide solutions: Evidence that chloroplast Photosystem I particles contain 4Fe4S centresBiochemical and Biophysical Research Communications, 1975
- Identification of a g = 1.90 high-potential iron-sulfur protein in chloroplastsBiochemical and Biophysical Research Communications, 1975
- Determination of the oxidation—reduction potential of the bound iron‐sulphur proteins of the primary electron acceptor complex of photosystem I in spinach chloroplastsFEBS Letters, 1974
- Quantitative studies on ferredoxin in greening bean leavesBiochemical Journal, 1973
- Development of Photochemical Activity and the Appearance of the High Potential Form of Cytochrome b-559 in Greening Barley SeedlingsPlant Physiology, 1973
- Quantitative EPR studies of the primary reaction of Photosystem I in chloroplastsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1972
- Primary Reactions of Photosynthesis: Photoreduction of a Bound Chloroplast Ferredoxin at Low Temperature as Detected by EPR SpectroscopyProceedings of the National Academy of Sciences, 1971