Increased expression of endothelin-1 gene in blood vessels of deoxycorticosterone acetate-salt hypertensive rats.
- 1 June 1993
- journal article
- abstracts
- Published by Wolters Kluwer Health in Hypertension
- Vol. 21 (6_pt_2) , 916-920
- https://doi.org/10.1161/01.hyp.21.6.916
Abstract
We have recently shown that the content of immunoreactive endothelin-1 is increased in acid extracts from blood vessels of deoxycorticosterone acetate (DOCA)-salt hypertensive rats compared with uninephrectomized control rats. We have also found by immunohistochemistry a significant increase in immunoreactive endothelin-1 in endothelial cells of aorta and mesenteric arteries of DOCA-salt hypertensive rats. In the present study, we investigated preproendothelin-1 gene expression in blood vessels of DOCA-salt hypertensive rats and uninephrectomized control rats. Northern blot analysis using a specific 32P-labeled complementary RNA probe for rat preproendothelin-1 of 319 base pairs revealed a fourfold to fivefold increase in abundance of preproendothelin-1 messenger RNA transcripts in both aorta and mesenteric arteries from DOCA-salt hypertensive rats. Thus, increased immunoreactive endothelin-1 content in blood vessels of DOCA-salt hypertensive rats is secondary to increased preproendothelin-1 gene expression. Exaggerated expression of the preproendothelin-1 gene in mineralocorticoid hypertension may contribute to the maintenance of elevated blood pressure.Keywords
This publication has 25 references indexed in Scilit:
- Increased endothelin-1 content in blood vessels of deoxycorticosterone acetate-salt hypertensive but not in spontaneously hypertensive rats.Hypertension, 1993
- Induction of endothelin-1 gene by angiotensin and vasopressin in endothelial cells.Hypertension, 1992
- Calcium, phosphoinositide, and 1,2-diacylglycerol responses of blood vessels of deoxycorticosterone acetate-salt hypertensive rats to endothelin-1.Hypertension, 1992
- Endothelin vascular receptors and responses in deoxycorticosterone acetate-salt hypertensive rats.Hypertension, 1992
- Plasma immunoreactive endothelin-1 in experimental malignant hypertension.Hypertension, 1991
- cDNA cloning, sequence analysis and tissue distribution of rat preproendothelin-1 mRNABiochemical and Biophysical Research Communications, 1991
- 1989 Corcoran lecture: adaptive and maladaptive responses of the arterial wall to hypertension.Hypertension, 1990
- Plasma concentrations of endothelin-1 in spontaneously hypertensive rats and DOCA-salt hypertensive ratsBiochemical and Biophysical Research Communications, 1990
- Hemodynamic shear stress stimulates endothelin production by cultured endothelial cellsBiochemical and Biophysical Research Communications, 1989
- Vascular angiotensin II receptors in renal and DOCA-salt hypertensive rats.Hypertension, 1983