Regulation of splicing: The importance of being translatable: FIGURE 1.
Open Access
- 17 December 2003
- journal article
- review article
- Published by Cold Spring Harbor Laboratory in RNA
- Vol. 10 (1) , 1-4
- https://doi.org/10.1261/rna.5112704
Abstract
RNA sequences that conform to the consensus sequence of 5′ splice sites but are not used for splicing occur frequently in protein coding genes. Mutational analyses have shown that suppression of splicing at such latent sites may be dictated by the necessity to maintain an open reading frame in the mRNA. Here we show that stop codon frequency in introns having latent 5′ splice sites is significantly greater than that of introns lacking such sites and significantly greater than the expected occurrence by chance alone. Both observations suggest the occurrence of a general mechanism that recognizes the mRNA reading frame in the context of pre-mRNA.Keywords
This publication has 9 references indexed in Scilit:
- The effect of nonsense codons on splicing: A genomic analysis: FIGURE 1.RNA, 2003
- Separable Roles for rent1/hUpf1 in Altered Splicing and Decay of Nonsense TranscriptsScience, 2002
- Conservation of an open-reading frame as an element affecting 5′ splice site selectionJournal of Structural Biology, 2002
- Alternatively Spliced TCR mRNA Induced by Disruption of Reading FrameScience, 2002
- Stop codons affect 5′ splice site selection by surveillance of splicingProceedings of the National Academy of Sciences, 2002
- Categorization and characterization of transcript-confirmed constitutively and alternatively spliced introns and exons from humanHuman Molecular Genetics, 2002
- Heat shock affects 5′ splice site selection, cleavage and ligation of CAD pre-mRNA in hamster cells, but not its packaging in InRNP particlesNucleic Acids Research, 1994
- G + C-rich tract in 5′ end of human intronsJournal of Molecular Biology, 1992
- Conserved Signals Around the 5′ Splice Sites in Eukaryotic Nuclear Precursor mRNAs: G-Runs are Frequent in the Introns and C in the Exons Near Both 5′ and 3′ Splice SitesJournal of Biomolecular Structure and Dynamics, 1989